Protocols

Analysis of 3D gene expression patterns in plants using whole-mount RNA in situ hybridisation

Frédérique Rozier, Vincent Mirabet, Teva Vernoux, and Pradeep Das

Nature Protocols (in press)

Analyse en 3 dimensions de l’expression des gènes


vizualisation of a 3D stack

The method allows the use of segmentation tools. The signal intensity is mapped on cell volumes.